The new MarrowMatic®
from ARROW Biotech
guarantees:
• maximum safety
• rapid and standardized
sample preparation
Marrowmatic
INTENDED USE
• Preparation of bone marrow aspirates
(defrustulation, disaggregation, micro- and macro-clot rupture for flow cytometric analysis o r other analyses requiring cell suspensions (e.g., cytogenetics, cell culture).
• Preparation of fine-needle aspirates, core
biopsies, or endoscopic "grab” biopsies (defrustulation and disaggregation) for flow cytometric analysis o r other analyses requiring cell suspensions (e.g., cytogenetics, cell culture).
INNOVATIVE FEATURES
• Operator safety. MarrowMatic performs the preparation procedures for bone marrow aspirates, core biopsies, and fine-needle aspirates for flow cytometric analysis (defrustulation, disaggregation, and filtration) fully automatically, without requiring sample manipulation with needles, scalpels, o r potentially dangerous devices.
• Time savings. Sample preparation takes about a minute, regardless o f the intrinsic quality o f the sample (presence of fragments, aggregates, clots, or hyperviscosity).
• Standardization o f results. The sample preparation method i s unique and produces a uniform product regardless of the treatment method.
• Optimization of cell recovery. MarrowMatic ensures optimal and standardized cell recovery without reducing cell viability.
• Prevention of technical problems o n subsequent analytical instruments (sample treatment with MarrowMatic reduces the formation of cell aggregates, which can cause
flow cytometry problems).
• Maintenance of sterility where required. MarrowMatic racks are available individually and sterile-packaged to also allow for cytogenetic investigations and/or cell culture setup.
HOW IT WORKS | 3 steps
Automated Procedure in about 1 minute
Choose the appropriate program: Bone Marrow Aspiration or Other Sampling (FNA / Core Biopsy / Endoscopic “Grab” Biopsy).
Place the mother tube (A) containing the sample in the rack and insert the special needle into the tube.
Insert the rack into the MarrowMatic and start the chosen procedure.
At the end of the procedure (about 1 minute), remove the rack and retrieve the daughter tube (B) with the de-stemmed and filtered sample. A cap for the daughter tube is included.
Dispose of the used rack in the appropriate containers provided by the laboratory for sharps and needles.
Comparison among MarrowMatic and usual manual procedures
MARROWMATIC AUTOMATED METHOD
✓ Automated dissociation + filtration
✓ Defrustulation, disaggregation and clot rupture
✓ Standardized, reproducible cell suspension
✓ Ready for analysis
MESH (MANUAL) METHOD
✗ Manual pushing through mesh
✗ Variable pressure and technique
✗ Cell loss due to adherence to mesh
✗ Inconsistent results, variable yields
✗ Risk of clogging and sample loss
✗ Time consuming
NEEDLE (MANUAL) METHOD
✗ Manual pipetting through needle
✗ Shear stress and cell damage
✗ Clot fragments may remain
✗ Operator dependent results
✗ Needle clogging risk
✗ Time consuming and labor intensive
Advantage of MarrowMatic
✓ Operator Safety
No manual manipulation with needles or potentially dangerous devices.
✓ Time Saving
Sample preparation in about 1 minute, regardless of sample quality.
✓ Standardization
Unique, fully automated process produces a uniform product every time.
✓ Optimized Cell Recovery
Maximum and reproducible cell recovery without compromising viability.
✓ Prevents Technical Issues
Reduces cell aggregates that can cause problems in flow cytometry.
✓ Maintains Sterility
Sterile, single-use racks available for cytogenetics and cell culture applications.
1. Select the program: bone marrow aspiration or other type of sampling (fine needle aspiration/needle biopsy)
2. Place the mother tube (A) in the designated rack slot and insert the special needle into the tube.
3. Insert the rack into the MarrowMatic and start the chosen procedure.
4. At the end of the procedure (about 1 minute), remove the rack from the
MarrowMatic and retrieve the daughter tube containing the de-stemmed and filtered sample (a cap for the daughter tube (B) is included with the rack).
5. Dispose of the used rack in the appropriate containers provided by the laboratory for sharps and needles.
RESULTS OBTAINED WITH THE MANUAL METHOD AND WITH MARROWMATIC
MarrowMatic sizes: L cm. 27,3, H cm. 40,4, P cm. 21, Weight 9 Kg
EXHIBIT A: Specifications
MarrowMatic Cod. 032600
The MarrowMatic is a patented device that works fully automatically using a specific disposable kit to defrustolate and filtrate the bone marrow samples providing a standardized bone marrow cells suspension. The MarrowMatic grants the total operator safety avoiding needles manipulation. The MarrowMatic provide time saving for the sample preparation making it ready in less than one minute.
• GLU (General Laboratory Use)
Works with MM-Kit
MM-Kit 1 Cod. 032601
Disposable kit made up of various tools to defrustolate the bone marrow sample.
Provided sterile.
It’s made up of:
HD/LD PE casing rack
Housing for original collected sample tube
Special homogenating needle
2 ml syringe
1 Empty 5 ml conical tube
100 micron cytostrain (100-98 Filcons)
MM-Kit 2 Cod. 032602
Disposable kit made up of various tools to dissociate and strain fine needle aspirate biopsies.
Provided sterile.
It’s made up of:
HD/LD PE casing rack
Housing for original collected sample tube
Special homogenating needle
2 ml syringe
2 Empty 5 ml conical tube
70 micron cytostrain (070-67 Filcons)
INNOVATION
Sede legale:
10138 Torino (TO)
Corso F. Ferrucci 46
Sede operativa:
10040 Rivalta (TO)
Via Carignano 65
P. Iva e cod. fisc. 13215210017